Research Intern (Downstream Processing) - Marlborough, MA

We are seeking a motivated and technically skilled intern (PhD level) to support research and development activities in downstream processing of extracellular vesicles (EVs) and viral vectors. The successful candidate will work closely with a multidisciplinary team to optimize and execute purification workflows using tangential flow filtration (TFF) and chromatography-based methods. This internship provides hands-on experience in biologics processing and analytical evaluation, contributing directly to process development for therapeutic nanoparticles. Onsite at our Marlborough, MA site, In the Research Intern role, you will serve as a critical contributor to the development and integration of Sartorius’ emerging technologies for characterization and purification of different types of nanoparticles such as extracellular vesicles and viral vectors. These leading-edge platforms will be utilized in basic research as well as the pharmaceutical and biotechnology industries. What you will accomplish together with us Execute Purification Protocols: Perform purification of EVs and viral vectors using TFF systems (hollow fiber and/or flat sheet) and various chromatography modalities (e.g., ion exchange, size exclusion, affinity). Optimize Unit Operations: Assist in the design and optimization of downstream steps to improve product yield, purity, and scalability. System Operation and Maintenance: Operate and maintain TFF and chromatography systems (e.g., AKTA or equivalent), including set-up, cleaning, and troubleshooting. Sample Handling and Quantification: Prepare and process samples following SOPs. Quantify particles using orthogonal methods such as NTA, qPCR, protein assays, and total RNA analysis. Process Monitoring and Data Recording: Monitor and document key parameters (e.g., transmembrane pressure, flux, recovery) during TFF runs. Maintain detailed lab notebooks and process logs. Analytical Support: Support integration of downstream data with analytical assays (e.g., f